England head coach Steve Borthwick has dropped veterans Billy Vunipola, Jonny May and Jack Nowell for his first Six Nations squad.
Borthwick has jettisoned three established performers under his predecessor Eddie Jones, who was sacked last month and has now been appointed Australia boss.
Saracens number eight Vunipola and wings May and Nowell were all involved in the 27-13 defeat by South Africa that concluded the autumn and spelt the end of the Jones era.
While the trio are not part of the 36-man squad that will next week begin preparations for the Six Nations opener against Scotland on February 4, there are recalls for Dan Cole and Elliot Daly.
Cole was dropped by England after the 2019 World Cup final while Daly has been frozen out since last year's Championship despite his superb form for Saracens.
Northampton's rookie fly-half Fin Smith is present for the first time, providing cover for Owen Farrell and Marcus Smith, while Ollie Hassell-Collins, George McGuigan, Cadan Murley and Jack Walker are the other uncapped players.
"This is an exciting squad with a blend of Six Nations experience and young talent and includes players who are in excellent form in the Premiership," Borthwick said.
"We are all looking forward to the challenges of the Six Nations and we will approach this great tournament with a spirit of courage and total commitment.
"I know the players can't wait to get back to Twickenham and give our fans a performance they can be proud of. The hard work for the Scotland game starts now."
England 36-man squad for the Six Nations, forwards: O Chessum (Leicester Tigers), D Cole (Leicester Tigers), B Curry (Sale Sharks), A Dombrandt (Harlequins), B Earl (Saracens), E Genge (Bristol Bears), J George (Saracens), J Heyes (Leicester Tigers), J Hill (Sale Sharks), N Isiekwe (Saracens), M Itoje (Saracens), C Lawes (Northampton Saints), L Ludlam (Northampton Saints), G McGuigan (Gloucester Rugby), B Rodd (Sale Sharks), S Simmonds (Exeter Chiefs), K Sinckler (Bristol Bears), M Vunipola (Saracens), J Walker (Harlequins), J Willis (Toulouse).
Backs: E Daly (Saracens), O Farrell (Saracens), T Freeman (Northampton Saints), O Hassell-Collins (London Irish), D Kelly (Leicester Tigers), M Malins (Saracens), J Marchant (Harlequins), A Mitchell (Northampton Saints), C Murley (Harlequins), H Slade (Exeter Chiefs), F Smith (Northampton Saints), M Smith (Harlequins), F Steward (Leicester Tigers), M Tuilagi (Sale Sharks), J van Poortvliet (Leicester Tigers), B Youngs (Leicester Tigers).
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here